BlueStar Prestained Protein Marker

he BlueStar Prestained Protein Ladder is a three color protein standard with 10 prestained proteins covering a wide range molecular weights from 10 to 180 kDa. The BlueStar Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximating the size of proteins. Broad range: 10 – 180 kDa High performance: 10 bands with 3 colours (blue, orange and green) Reference bands: 28 kDa (green) and 75 kDa (orange) Sharp bands Ready to use: Supplied in a loading buffer for direct loading Storage Buffer 20 mM Disodium hydrogen phosphate pH 8.0 (at 25°C ), 2 % SDS, 0.2% Triton X-100,10 mM 2-Mercaptoethanol, 6 M Urea and 27% (v/v) Glycerol. Storage Stable at -20°C for 12 months. Stable at +4°C for 3 months. Recommendations for Loading 1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil. 2. Mix thoroughly, to ensure that the solution is homogeneous. 3. Load the following volumes of the ladder on SDS-polyacrylamide gel: 5 μl per well for mini-gels, 3 μl per well for blots 10 μl per well for large gels, 6 μl per well for blots Applications • Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis • Monitoring of protein transfer onto membranes during Western blotting • Sizing of proteins on SDS-polyacrylamide gels and Western blots