High-Speed Plasmid Mini Kit (PD100, PD300)

The High-Speed Plasmid Mini Kit was designed for both plasmid and cosmid DNA purification. This plasmid kit will purify plasmid DNA/cosmid DNA from 1-5 ml of cultured bacterial cells using an efficient plasmid miniprep system. The High-Speed Plasmid Mini Kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer, TE or water. Typi

cal yields are 20-30 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells. This plasmid kit does not require DNA phenol extraction or alcohol precipitation and the entire procedure can be completed within 15 minutes.

Specifications (Cat. # PD100, PD300)

• Purify plasmid DNA or cosmid DNA within 15 minutes!
• High Yield: up to 30 μg of pure plasmid DNA or cosmid DNA
• Plasmid Size: 1-15 kb
• Sample Volume: 1-5 ml of bacterial cells
• Spin Columns: glass fiber membrane optimized for plasmid DNA purification
• Elution Volume: 30-100 µl
• Storage: dry at room temperature (15-25ºC)

APPLICATIONS

Restriction Enzyme Digestion, Library Screening, Ligation, PCR, Transformation and Sequencing Reactions

COMPONENTS

• PD1, PD2, PD3 Buffers
• W1 Buffer
• Wash Buffer
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
• RNase A
• PD Columns
• 2 ml Collection Tubes

QUALITY CONTROL

The quality of the High-Speed Plasmid Mini Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 4 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the High-Speed Plasmid Mini Kit. Following the purification process, a yield of more than 20 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1 µg) is used in EcoRI digestion, and checked by electrophoresis.