EcoExtra™ Magnetic Beads Plasmid Kit (MPD192, MPD576, MPD1920)

The EcoExtra™ Magnetic Beads Plasmid Kit was designed for high throughput purification of plasmid DNA from up to 16 ml of cultured bacterial cells per reaction using an efficient magnetic beads system. A modified alkaline lysis method and RNase treatment a re used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 6-10 μ g for high copy number plasmid from 1 ml of cultured E. coli cells. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions. More important, the EcoExtra™ series kits enable up to 80% reduction of plastic material waste compared to silica-column based kits. Make lab works a lot more eco friendly.

Specifications (Cat. # MPD192, MPD576, MPD1920)

• Sample Volume: 1-16 ml of cultured bacterial cells
• High Yield: 6-10 μg for high-copy number plasmid from 1 ml of cultured E. coli cells
• High Purity plasmid DNA: A260/A280 =1.8-2.0, A260/230 =2.0-2.4
• Plasmid DNA Size: 1-20 kb
• Elution volume: 25-100 µl
• Manual or automated plasmid DNA isolation
• Reduce up to 80% of plastic material waste compared to spin-column based kits, more eco friendly!
• Storage: dry at room temperature (15-25°C)

APPLICATIONS

Restriction Enzyme Digestion, Library Screening, Ligation, PCR, Transformation and Sequencing Reactions

COMPONENTS

• MPD1 Buffer
• MPD2 Buffer
• MPD3 Buffer
• Wash Buffer
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
• RNase A
• MPD Magnetic Beads

QUALITY CONTROL

The quality of the EcoExtra™  Magnetic Beads Plasmid Kit  is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 4 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript is purified using the EcoExtra™  Magnetic Beads Plasmid Kit . Following the purification process, a yield of more than 30 μg is obtained and the A260/A280 ratio is between 1.8-2.0.