Presto™ Rapid Extract PCR kit (RPCR100, RPCR500)

Presto™ Rapid Extract PCR kit consists of ZEROprep™ DNA Reagent and 2X Hot Start Taq Master Mix with Dye. ZEROprep™ DNA Reagent is designed for efficient release of DNA from variety of samples for direct use in PCR reactions without purification. In addition, 2X Hot Start Taq PCR Master Mix with Dye includes all of the components necessary to perform PCR. Add primers, template DNA and sterile water to complete the PCR reaction mix in routine PCR assays. Simply place the samples in the ZEROprep™ DNA reagent, only 10 minutes incubation and transfer the sample lysate as DNA template to 2X Hot Start Taq Master Mix with Dye for PCR assay. After PCR reaction, the PCR mixture could be conveniently loaded on the agarose gel without mix with loading dye in advance.

Advantages (Cat. RPCR100, RPCR500)

• Wide variety of sample types (tissue, blood, plant, bacteria, yeast/fungus, virus)
• Use DNA directly in PCR reactions
• Ready-to-use 2X Hot Start Taq Master Mix with DNA loading dye
• Recombinant sourced Hot Start Taq DNA Polymerase
• Fast PCR set up due to 2-8 ºC storage temperature which eliminates thawing time
• Amplification of long targets: 100 bp – 5 kb
• 3' – 5' Exonuclease Proofreading Ability: NO
• 5' – 3' Exonoclease Activity: YES

SOURCE

The Hot Start Taq DNA Polymerase (Antibody modification) is a hot-start thermostable DNA polymerase which was expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.

COMPOSITION

• ZEROprep™ DNA Reagent
• 2X Hot Start Taq Master Mix with Dye

UNIT DEFINITION

One unit incorporates 10 nmol of dNTP into acid insoluble material in 30 minutes at 74ºC.

QUALITY CONTROL

Presto™ Rapid Extract PCR kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. DNA from a 1 mg tissue sample is lysed in ZEROprep™ DNA Reagent. A 2 μl aliquot of lysate is added directly into a 25 μl PCR mix using 2X Hot Start Taq PCR Master Mix with Dye.